ABSTRACT
This research presents a virtual tour performed on the oppidum of Ulaca, one of the most relevant archaeological sites of the Iberian Peninsula during the Late Iron Age (ca. 400–50 BC). Beyond the clear benefits of the tool to the interpretation, dissemination, and knowledge of the mentioned archaeological site and its surroundings, the novelty of this research is the implementation of the platform in alternative scenarios and purposes. In this way, the present work verifies how the access to multi-source and spatially geolocated information in the same tool (working as a geospatial database) allows the promotion of cross-sectional investigations in which different specialists intervene. This peculiarity is also considered useful to promote tourism with an interest beyond the purely historical/archaeological side. Likewise, the possibility of storing and managing a large amount of information in different formats facilitates the investigation in the contexts of excavations and archaeological or environmental works. In this sense, the use of this kind of tool for the study of cultural landscapes is especially novel. In order to better contextualize the potential of the virtual tour presented here, an analysis about the challenges and possibilities of implementing this tool in environments such as the Ulaca oppidum is performed. The selected site stands out for: (i) being in a unique geological, environmental and ecological context, allowing us to appreciate how human beings have modified the landscape over time;(ii) presenting numerous visible archaeological remains with certain conservation problems;and (iii) not having easy access for visitors.
ABSTRACT
El objetivo del trabajo fue explorar los factores que inciden en la puesta en práctica de la educación a distancia en el contexto pandémico, a nivel global. Metodológicamente, se hizo una revisión sistemática sobre la productividad científica en torno a la variable de estudio, en las bases de datos Education Resouces Information Center, ScienceDirect, Scopus y Web of Science. Siendo criterios de inclusión artículos de investigación publicados en revistas científicas en el período 2020-2021, escritos en español e inglés en el área de educación. Los principales hallazgos mostraron datos empíricos y teóricos referentes a: Dificultad para: la conectividad a internet, acceder a equipos e infraestructura tecnológica y digital y relacionarse estudiantes-docentes;también se encontró débiles competencias digitales;amplitud de la brecha digital y en las desigualdades educativas y socioeconómicas. Se concluye, que la tendencia ha sido el incremento en el uso de la tecnología de información y comunicación para sostener las actividades educativas, pero paralelamente el acceso a estos medios es limitado, conllevando a complejos procesos educativos bajo la modalidad a distancia, que exigen ampliar la búsqueda de estrategias que minimicen las dificultades impuestas por la realidad, siendo la alternativa la modalidad hibrida.
ABSTRACT
BACKGROUNDRecent studies have reported T cell immunity to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in unexposed donors, possibly due to crossrecognition by T cells specific for common cold coronaviruses (CCCs). True T cell crossreactivity, defined as the recognition by a single TCR of more than one distinct peptide-MHC ligand, has never been shown in the context of SARS-CoV-2.METHODSWe used the viral functional expansion of specific T cells (ViraFEST) platform to identify T cell responses crossreactive for the spike (S) glycoproteins of SARS-CoV-2 and CCCs at the T cell receptor (TCR) clonotype level in convalescent COVID-19 patients (CCPs) and SARS-CoV-2-unexposed donors. Confirmation of SARS-CoV-2/CCC crossreactivity and assessments of functional avidity were performed using a TCR cloning and transfection system.RESULTSMemory CD4+ T cell clonotypes that crossrecognized the S proteins of SARS-CoV-2 and at least one other CCC were detected in 65% of CCPs and unexposed donors. Several of these TCRs were shared among multiple donors. Crossreactive T cells demonstrated significantly impaired SARS-CoV-2-specific proliferation in vitro relative to monospecific CD4+ T cells, which was consistent with lower functional avidity of their TCRs for SARS-CoV-2 relative to CCC.CONCLUSIONSOur data confirm, for what we believe is the first time, the existence of unique memory CD4+ T cell clonotypes crossrecognizing SARS-CoV-2 and CCCs. The lower avidity of crossreactive TCRs for SARS-CoV-2 may be the result of antigenic imprinting, such that preexisting CCC-specific memory T cells have reduced expansive capacity upon SARS-CoV-2 infection. Further studies are needed to determine how these crossreactive T cell responses affect clinical outcomes in COVID-19 patients.FUNDINGNIH funding (U54CA260492, P30CA006973, P41EB028239, R01AI153349, R01AI145435-A1, R21AI149760, and U19A1088791) was provided by the National Institute of Allergy and Infectious Diseases, the National Cancer Institute, and the National Institute of Biomedical Imaging and Bioengineering. The Bloomberg~Kimmel Institute for Cancer Immunotherapy, The Johns Hopkins University Provost, and The Bill and Melinda Gates Foundation provided funding for this study.